研究業績 (Publications)

icon 原著論文 (Original papers)
icon 欧文総説 (Reviews)
icon 国際学会紀要 (Proceedings)
icon 日本語総説 (Reviews (Japanese))


 Google Scholar Citations
 ORCID  Scopus  Publons  *corresponding author

45. Ken Motohashi*
"A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products"
Sci. Rep. 9, 6417 (2019)

44. Hokuto Nakayama, Tomoaki Sakamoto, Yuki Okegawa, Kaori Kaminoyama, Manabu Fujie, Yasunori Ichihashi, Tetsuya Kurata, Ken Motohashi, Ihsan Al-Shehbaz, Neelima Sinha and Seisuke Kimura*
"Comparative transcriptomics with self-organizing map reveals cryptic photosynthetic differences between two accessions of North American Lake cress"
Sci. Rep. 8, 3302 (2018)

43. Ken Motohashi*
"Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods"
Biochem. Biophys. Rep. 9, 310-315 (2017)

42. Ken Motohashi*
"Seamless ligation cloning extract (SLiCE) method using cell lysates from laboratory Escherichia coli strains and its application to SLiP site-directed mutagenesis"
Methods Mol. Biol. 1498, 349-357 (2017)

41. Yuki Okegawa and Ken Motohashi*
"Expression of spinach ferredoxin-thioredoxin reductase using tandem T7 promoters and application of the purified protein for in vitro light-dependent thioredoxin-reduction system"
Protein Expr. Purif. 121, 46-51 (2016)

40. Yuki Okegawa1, Masanori Koshino1, Teruya Okushima and Ken Motohashi*
"Application of preparative disk gel electrophoresis for antigen purification from inclusion bodies"
Protein Expr. Purif. 118, 77-82 (2016)
1These authors contributed equally to this work.

39. Yuki Okegawa and Ken Motohashi*
"Chloroplastic thioredoxin m functions as a major regulator of Calvin cycle enzymes during photosynthesis in vivo"
Plant J. 84, 900-913 (2015)

38. Yuki Okegawa and Ken Motohashi*
"A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit"
Biochem. Biophys. Rep. 4, 148-151 (2015)

37. Yuki Okegawa and Ken Motohashi*
"Evaluation of seamless ligation cloning extract (SLiCE) preparation methods from an Escherichia coli laboratory strain"
Anal. Biochem. 486, 51-53 (2015)


36. Ken Motohashi*
"A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis"
BMC Biotechnol. 15, 47 (2015)

35. Ken Motohashi*1 and Yuki Okegawa1
"Method for enhancement of plant redox-related protein expression and its application for in vitro reduction of chloroplastic thioredoxins"
Protein Expr. Purif. 101, 152-156 (2014)
1These authors contributed equally to this work.

34. Keisuke Yoshida, Ko Noguchi, Ken Motohashi and Toru Hisabori*

"Systematic exploration of thioredoxin target proteins in plant mitochondria"
Plant Cell Physiol.   54, 875-892 (2013)

33. Ken Motohashi* and Toru Hisabori
"CcdA is a thylakoid membrane protein required for the transfer of reducing equivalents from stroma to thylakoid lumen in the higher plant chloroplast"
Antioxid. Redox Signal.   13, 1169-1176 (2010)


32. Kouji Kojima, Ken Motohashi, Takuya Morota, Masaru Oshita, Toru Hisabori, Hidenori Hayashi and Yoshitaka Nishiyama*
"Regulation of translation by the redox state of elongation factor G in the cyanobacterium Synechocystis sp. PCC 6803"
J. Biol. Chem.  284, 18685-18691 (2009)

31. Ken Motohashi, Patrick G. N. Romano and Toru Hisabori*
"Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin"
Methods Mol. Biol.  479, 117-131 (2009)

30. Koichi Kobayashi, Nobuyoshi Mochizuki, Naho Yoshimura, Ken Motohashi, Toru Hisabori and Tatsuru Masuda*
"Functional analysis of Arabidpsis thaliana isoforms of the Mg-chelatase CHLI subunit"
Photochem. Photobiol. Sci.  7, 1188-1195 (2008)

29. Shoko Hishiya, Wakako Hatakeyama, Yoko Mizota, Naomi Hosoya-Matsuda, Ken Motohashi, Masahiko Ikeuchi and Toru Hisabori*
"Binary reducing equivalent pathways using NADPH-thioredoxin reductase and ferredoxin-thioredoxin reductase in the cyanobacterium Synechocystis sp. strain PCC6803"
Plant Cell Physiol.  49, 11-18 (2008)


28. Masahiko Wakita, Shinji Masuda*, Ken Motohashi, Toru Hisabori, Hiroyuki Ohta and Ken-ichiro Takamiya
"The significance of typeII and PrxQ peroxiredoxins for antioxidative stress response in the purple bacterium rhodobacter sphaeroides"
J. Biol. Chem.  282, 27792-27801 (2007)

27. Akinori Ikegami, Naho Yoshimura, Ken Motohashi, Shigekazu Takahashi, Patrick G. N. Romano, Toru Hisabori, Ken-ichiro Takamiya and Tatsuru Masuda*
"The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin"
J. Biol. Chem.  282, 19282-19291 (2007)

26. Ken Motohashi and Toru Hisabori*
"HCF164 receives the reducing equivalents from stroma thioredoxin across thylakoid membrane and mediates reduction of target proteins in thylakoid lumen"
J. Biol. Chem. 281, 35039-35047 (2006)

25. Satoshi Hara, Ken Motohashi, Fumio Arisaka, Patrick G. N. Romano, Hosoya-Matsuda, Nobuhiro Kikuchi, Naoki Fusada, and Toru Hisabori*
"Thioredoxin-h1 reduces and reactivates the oxidized cytosolic malate dehydrogenase dimer in higher plants"

J. Biol. Chem. 281, 32065-32071 (2006)

24. Yoshiki Yamaryo, Ken Motohashi, Ken-ichiro Takamiya, Toru Hisabori and Hiroyuki Ohta*
"In vitro reconstitution of monogalactosyldiacylglycerol (MGDG) synthase regulation by thioredoxin"
FEBS Lett.  580, 4086-4090 (2006)

23. Naomi Hosoya-Matsuda, Ken Motohashi, Hidehisa Yoshimura, Akiko Nozaki, Kazuhito Inoue, Masayuki Ohmori and Toru Hisabori*
"Anti-oxidative stress system in cyanobacteria: Significance of type II peroxiredoxin and the role of 1-Cys peroxiredoxin in Synechocystis sp. strain PCC 6803" 
J. Biol. Chem. 280, 840-846 (2005)

22. Hanayo Ueoka-Nakanishi, Yoichi Nakanishi, Hiroki Konno, Ken Motohashi, Dirk Bald and Toru Hisabori*
“Inverse regulation of rotation of F1-ATPase by the mutation at the regulatory region on the gamma subunit of chloroplast ATP synthase" 
J. Biol. Chem. 279, 16272-16277 (2004)

21. Daisuke Yamazaki1, Ken Motohashi1, Takeshi Kasama, Yukichi Hara and Toru Hisabori*
"Target proteins of the cytosolic thioredoxins in Arabidopsis thaliana"
Plant Cell Physiol. 45, 18-27 (2004)
1
These authors contributed equally to this work.

20. Ken Motohashi, Fumie Koyama, Yoichi Nakanishi, Hanayo Ueoka-Nakanishi and Toru Hisabori*
"Chloroplast cyclophilin is a target protein of thioredoxin: Thiol modulation of the peptidyl-prolyl cis-trans isomerase activity" 
J. Biol. Chem.
278, 31848-31852 (2003)

19. Hisayoshi Makyio, Hajime Niwa, Ken Motohashi, Hideki Taguchi and Masasuke Yoshida*
"Stabilization of FtsH-unfolded protein complex by binding of ATP and blocking of protease"
Biochem. Biophys. Res. Commun. 296, 8-12 (2002)

18. Yo-hei Watanabe, Ken Motohashi and Masasuke Yoshida*
"Roles of the two ATP-binding sites of ClpB from Thermus thermophilus"
J. Biol. Chem.  277, 5804-5809 (2002)

17. Ken Motohashi1, Aiko Kondoh1, Michael T. Stumpp and Toru Hisabori*
"Comprehensive survey of novel proteins targeted by chloroplast thioredoxin"
Proc. Natl. Acad. Sci. USA  98, 11224-11229 (2001)
1
These authors contributed equally to this work.

16. Hiroki Konno, Masahide Yodogawa, Michael T. Stumpp, Peter Kroth, Heinrich Strotmann, Ken Motohashi, Toyoki Amano and Toru Hisabori*
"Inverse regulation of F1-ATPase activity by a mutation at the regulatory region on the g subunit of chloroplast ATP synthase"
Biochem. J.  352, 783-788 (2000)

15. Yo-hei Watanabe, Ken Motohashi, Hideki Taguchi and Masasuke Yoshida*"Heat-inactivated proteins managed by DnaKJ-GrpE-ClpB chaperones are released as a chaperonin-recognizable non-native form"
J. Biol. Chem. 275, 12388-12392 (2000)

14. Yuko Asahara, Kyoko Atsuta, Ken Motohashi, Hideki Taguchi, Masafumi Yohda and Masasuke Yoshida*
"FtsH recognizes proteins with unfolded structure and hydrolyzes the carboxyl side of hydrophobic residues"
J. Biochem.
127, 931-937 (2000)

13. Kiyoshi Ozawa, Takanori Meikari, Ken Motohashi, Masasuke Yoshida and Hideo Akutsu*
"Evidence for the presence of an F-type ATP synthase involved in sulfate respiration in Desulfovibrio vulgaris" 
J. Bacteriol. 182, 2200-2206 (2000)

12. Ken Motohashi, Satomi Shibata, Yukio Ozaki, Yutaka Yatomi and Yasuyuki Igarashi*
"Identification of lysophospholipid receptors in human platelets: the relation of two agonists, lysophosphatidic acid and sphingosine 1-phosphate"
FEBS Lett. 468, 189-193 (2000)

11. Yasuyuki Kato-Yamada, Dirk Bald, Mamiko Koike, Ken Motohashi, Toru Hisabori and Masasuke Yoshida*
"
e subunit, an endogenous inhibitor of bacterial F1-ATPase, also inhibits F0F1-ATPase"
J. Biol. Chem. 274, 33991-33994 (1999)

10. Ken Motohashi, Yohei Watanabe, Masafumi Yohda and Masasuke Yoshida*
"Heat-inactivated proteins are rescued by the DnaK•J-GrpE set and ClpB chaperones"
Proc. Natl. Acad. Sci. USA  96, 7184-7189 (1999)

9. Michael T. Stumpp, Ken Motohashi and Toru Hisabori*
"Chloroplast thioredoxin mutants without active site cysteines facilitate the reduction of the regulatory disulfide bridge on the g subunit of chloroplast ATP synthase"
Biochem. J. 341, 157-163 (1999)

8. Motoki Kanekatsu, Hitoshi Saito, Ken Motohashi and Toru Hisabori
"The b subunit of chloroplast ATP synthase (CFoCF1-ATPase) is phosphorylated by casein kinase II"
Biochem. Mol. Biol. Int. 46, 99-105 (1998)

7. Toru Hisabori*, Ken Motohashi, Peter Kroth, Heinrich Strotmann and Toyoki Amano
"The formation or the reduction of a disulfide bridge on the
g subunit of chloroplast ATP synthase affects the inhibitory effect of the e subunit"
J. Biol. Chem. 273, 15901-15905 (1998)

6. Ken Kitano, Ken Motohashi, Masasuke Yoshida and Kunio Miki*
"A novel approach to crystallizing proteins with temperature induction method: GrpE protein from Thermus thermophilus"
J. Cryst. Growth   186, 456-460 (1998) 

5. Ken Motohashi, Masafumi Yohda, Masafumi Odaka and Masasuke Yoshida*
"K+ is an indispensable cofactor for GrpE stimulation of ATPase activity of DnaK•DnaJ complex from Thermus thermophilus"
FEBS Lett.  412, 633-636 (1997)

4. Toru Hisabori*, Yasuyuki Kato, Ken Motohashi, Peter Kroth-Pancic, Heinrich Strotmann and Toyoki Amano
"The regulatory functions of the g and e subunits from chloroplast CF1 are transferred to the core complex, a3b3, from thermophilic bacterial F1"
Eur. J. Biochem.
  247, 1158-1165 (1997)

3. Ken Motohashi, Masafumi Yohda, Isao Endo and Masasuke Yoshida*
"A novel factor required for the assembly of the DnaK and DnaJ chaperones of Thermus thermophilus"
J. Biol. Chem.  271, 17343-17348 (1996)

2. Ken Motohashi, Hideki Taguchi, Noriyuki Ishii and Masasuke Yoshida*
"Isolation of the stable hexameric DnaK•DnaJ complex from Thermus thermophilus" 
J. Biol. Chem.  269, 27074-27079 (1994)

1. Yuji Murakami, Ken Motohashi, Kazuyoshi Yano, Kazunori Ikebukuro, Kenji Yokoyama, Eiichi Tamiya and Isao Karube*
"Micromachined electroporation system for transgenic fish" 
J. Biotechnol.
  34, 35-42 (1994)

 -------------------------------------------
[欧文総説 (Reviews)]
2. Toru Hisabori*, Ken Motohashi, Naomi Hosoya-Matsuda, Hanayo Ueoka-Nakanishi, Patrick G. N. Romano
“Towards a functional dissection of thioredoxin networks in plant cells”
Photochem. Photobiol.  83, 145-151 (2007)

1. Toru Hisabori*, Satoshi Hara, Tetsufumi Fujii, Daisuke Yamazaki, Naomi Hosoya-Matsuda and Ken Motohashi
“Thioredoxin affinity chromatography: for further understanding on the thioredoxin network”
J. Exp. Bot. 416, 1463-1468 (2005)


 -------------------------------------
国際学会紀要 (Proceedings)]
6. Ken Motohashi and Toru Hisabori
“Functional analysis of HCF164, a thioredoxin-like protein in the thylakoid lumen”
In Energy from the Sun, p935-938, Springer (2008)

5. Ken Motohashi, Fumie Koyama, Yoichi Nakanishi, Hanayo Ueoka-Nakanishi, Masasuke Yoshida and Toru Hisabori
“Target proteins of chloroplast thioredoxins –Efficient survey and the confirmation” In Photosynthesis: Fundamental Aspects to Global Perspectives, pp.671-673, Allen Press, USA (2005)

4. Toru Hisabori, Hanayo Ueoka-Nakanishi, Yoichi Nakanishi, Dirk Bald, Ken Motohashi, Masasuke Yoshida and Hiroki Konno
“The principle of redox regulation of chloroplast ATPase activity”
In Photosynthesis: Fundamental Aspects to Global Perspectives,
pp.3-5, Allen Press, USA (2005)

3. Yuhei Tsuchida, Tsuyoshi Furumoto, Ken Motohashi, Toru Hisabori and Katsura Izui
“Biochemical evidence of redox regulation of phosphoenolpyruvate carboxylase kinase”
In Photosynthesis: Fundamental Aspects to Global Perspectives, pp.674-675, Allen Press, USA (2005)

2. Yoshiki Yamaryo, Ken Motohashi, Tatsuru Masuda, Hiroshi Shimada, Ken-ichiro Takamiya and Hiroyuki Ohta
“Galactolipid synthesis coordinates with the expression of photosynthetic activity via redox cascade”
In Advanced Research on Plant Lipids, Murata, N., Yamada, M., Nishida, I., Okuyama, H., Sekiya, J., Hajime, W. eds, pp 207-210, Kluwer Academic Publishers, the Netherland (2003)

1. Toru Hisabori, Ken Motohashi, Mamiko Koike, Peter Kroth, Heinrich Strotmann and Toyoki Amano
“The relation between the disulfide bridge of the g subunit of CF1 and the inhibitory effects of the e subunit”
In Photosynthesis:Mechanism and Effects, Grab, G. eds, pp1711-1714, Kluwer Academic Publishers, the Netherland (1998)

  -------------------------------------------
[日本語総説 (Reviews (Japanese))]
7.桶川友季、本橋健
“チオレドキシンシステムによる光合成の調節機構 ~変動する光環境で植物はどのように効率良く光合成を行っているのか?~”
化学と生物 56, 452-453 (2018)

6.本橋健
“シームレスクローニング法 ~古典的な制限酵素とDNAリガーゼを用いないクローニング~”
生物工学 96, 20-24 (2018)

5.本橋健

SLiCE法:精製酵素をいっさい使わない"超低コスト"シームレスクローニング
実験医学 34, 2349-2354 (2016)
Application of seamless ligation cloning extract (SLiCE) from laboratory Escherichia coli strains to seamless DNA-cloning

4.本橋健

高等植物葉緑体におけるレドックス制御~チラコイド内腔におけるレドックス調節機構について~
光合成研究 20, 4-8 (2010)
Redox modulation system in thylakoid lumen side of higher plant chloroplasts

3.本橋健、久堀徹
酸化還元により制御されるチオレドキシン標的タンパク質の探索~研究の進展とこれからの課題~
細胞工学 25, 409-413 (2006)
Comprehensive screening of thioredoxin target proteins: development of effective methodologies and approaches for their biochemical analysis

2.久堀徹、本橋健、中西洋一、小山史恵
網羅的でない、でもインテリジェントなプロテオミクス研究を求めて
蛋白質・核酸・酵素 48, 1263-1268 (2003)
"Intelligent proteomics: requirement of new strategy

1.田口英樹、本橋健、吉田賢右
分子シャペロン
蛋白質・核酸・酵素 39, 1233-1240 (1994)
"Molecular Chaperone