Professor Akihiko Maeda
■Area and Subject Taught：Zoonosis, Public health
■Research Theme(s)：Molecular virology and molecular epidemiology of mosquito-borne flaviviruses
■Academic Degrees：D.V.M., Ph.D.
■Keywords for Research Field：Virology, Epidemiology, Zoonosis
[Research Overview]
I am a virologist. My main research area is molecular virology and molecular epidemiology of viral zoonotic diseases, especially mosquito-borne flaviviruses diseases. Mosquito-borne flaviviruses cause severe fever, encephalitis, and hemorrhagic fever in human and animals. The diseases are major public concern all over the world due to recent global warming. My current research themes are (1) molecular characterization of mosquito-borne flaviviruses infection to figure out host-virus interuction, and (2) analysis of virus ecology in nature to understand how the viruses outbreak in human and animals.
[Notable Publications and Works]
1. Murata, R., Eshita, Y., Maeda, A., Maeda, J., Sakai, A., Tanaka, T., Yoshii, K., Kariwa, H., Umemura, T., and Takashima, I. “Glycosylation of West Nile virus envelope protein increases In vivo and In vitro viral multiplication in birds.” American Journal of Tropical Medicine and Hygiene, 82: 696-704, 2010
2. Hasebe, R., Suzuki, T., Makinno, Y., Igarashi, M., Yamanouchi, S., Maeda, A., Horiuchi, M., Sawa, H., and Kimura, T. “Transcellular transport of West Nile virus-like particles across human endothelial cells depends on residues 156 and 159 of envelope protein.” BMC Microbiology, 10: 165-164., 2010
3. Moritoh, K., Maeda, A., Sasaki, N., and Agui, T. “Deveropement and application of West Nile virus subgenomic replicon RNA expressing secreted alkarine phosphatase.” The Journal of Veterinary Medical Science, In press, 2010
4. Ma, H., Ke, C.-W, Maeda, J., Takashima, I., Kurane, I., and Maeda, A. “Epidemiological study of flaviviruses, in Guangdong province, China, 2005-2007.” In “Animal viruses” Ed. Maeda, A., pp. 89-102. Trans Research Network, Kerala, India, 2010
5. Maeda, A., Maeda, J., Murata, R., Akiyama, M., Kariea, H., Takashima, I., and Kurane, I. “Differential sero-diagnosis of flaviviruses using subviral particles and virus-like particles.” In “Animal viruses” Ed. Maeda, A., pp. 103-116. Trans Research Network, Kerala, India, 2010
6. Maeda, A., Murata, R., Akiyama, M., Takashima, I., Kariwa H., Watanabe, T., Kurane, I., and Maeda, J. A PCR-based protocol for the generation of a recombinant West Nile virus. Virus Research, 144: 35-43, 2009
7. Zheng, K., Zhou, H.-Q., Yan, J., Ke, C.-W. , Maeda, A., Maeda, J., Takashima, I., Kurane, I., Ma, H., and Xie, X.-M. Molecular characterization of the E gene of dengue virus type 1 isolated in Guangdong Province, China, in 2006., Epidemiology and Infection, 137: 73-78, 2009
8. Abu Daud, N.H., Kariwa, H., Tkachenko, E., Dzagurnova T., Medvedvedkina, O., Tkachenko, P., Ishizika M., Seto, T., Miyashita D., Sanada T., Nakauchi, M., Yoshii, K., Maeda A., Yoshimatsu, K. Arikawa, J., and Takashima I. Genetic and antigenetic analyses of a Puumala virus isolate as a potential vaccine strain. Japanese Journal of Veterinary Research, 56: 151-165, 2008
9. Nakauchi, M., Kariwa, H., Kon, Y., Yoshii, K., Maeda, A., and Takashima, I. Analysis of Severe Acute Respiratory Syndrome Coronavirus Structural Proteins in Virus-Like Particle Assembly. Microbiology and Immunology, 52: 625-630, 2008
10. Maeda, J., Takagi, H., Hashimoto, S., Kurane, I., and Maeda, A. A PCR-based protocol for generating West Nile virus replicons. Journal of Virological Methods, 148: 244-252, 2008
11. Maeda, A., Maeda, J., Takagi, H., and Kurane, I. Detection of small RNAs containing the 5’- and the 3’-end sequences of viral genome during West Nile virus replication. Virology, 371: 130-138, 2008