1. Study for tobacco smoke
Cigarette smoke is a major risk factor for pulmonary diseases. Alveolar
macrophages (AM) phagocytize microorganisms, produce reactive oxygen species
(ROS) and play an important role in immunological surveillance for the
lung. In previous studies, we demonstrated tobacco smoking inhibits immune
functions in AM. However, the mechanism of inhibition of immune function
in AM is not well defined. In the aim of our study, we are investigating
the suppressive mechanism of immune functions in AM associated with DNA
damage by cigarette smoke exposure.
Cigarette smoke (CS) consists of mainstream smoke,
sidestream smoke, growing smoke, diffusion smoke, effusion smoke, smoldering
smoke and blowing smoke, which are exhaled by a smoker.About 6000 kinds of chemical substances are contained in the cigarette
smoke, and it is separated from a gas phase and
a particles phase by the condition of the material. These contain many
kind of carcinogens, injury materials of epithelial cells of lung
We are focusing on cigarette smoke, which is currently attracting attentions as an environmental problem, hence are investigating to elucidate the
effect of smoking on the relationship between smoke and pulmonary immune
cells, particulary alveolar macrophages, and pulmonary epithelial cells.
Smoking has been shown to to increase production of active oxygen by alveolar
macrophages, which induce DNA damage in these cells, and it has also been
demonstrated that their immune functions such as antigen presentation and
cytokine production are impaired. It has been suggested that these inhibitions
in immune functions are related to the incidence of smoking-related pulmonary
epithelial cancer and tumor cell proliferation, and we are investigating
this relationship and gmaking a science for smoking.h Since the fact that
immune functions are suppressed by smoking and tumor growth, we are also
investigating the mechanisms whereby immunoenhancing substances may act
to restore suppressed immune functions.
Wellcome to Takeuchi Lab.
Exposure of main stream cigarette smoke to mice
2. Study for Natural products
Jungle honey (JH) is collected from timber and blossom
by wild honey bees that live in the tropical forest of Nigeria.
This is used as traditional medicine for cold, skin inflammation and burn wound
but not only health care. However, the effect of Jungle honey on
immunomodulatory activity is not yet found clearly. We have previously reported
the effect of natural components on immune system. Therefore, we are
investigating the effect of jungle honey on immune system and anti-tumor
activity using mice.
(2) Agaricus Blazei Murill
Agaricus blazei Murill (ABM)
has been traditionally used as medicine in Brazil. ABM has been reported for
anti-tumor activity and immune activity. It is unclear how ABM extract activates
the immune system and anti-tumor activity. We have demonstrated that extract of
Agaricus blazei Murill (ABM) activated immune functions in mice. However, the
mechanism of the activity of immune functions and ant-tumor activity by ABM is
not well defined. Therefore, we are focusing on immune cells functions
associated with anti-tumor activity by ABM hot water extract and its characterization
of effective component.
Agaricus blazei Murill that
belong to the basidiomycetes can grow under special weather condition( daytime
35 , night 20-25) and humidity. Although it is generally accepted that Agaricus blazei
Murill possess anti-tumor activity, the detail of immune activity is unclear.
Agaricus blazei Murill
Natural products are known to have biological activity, and we have previously
investigated the effect of natural products on immune function.. Honey
contains various vitamins, minerals and amino acids as well as glucose
and fructose and is popular as a natural food.. There is a wide variety
of honey (Manuka honey, Pasture honey, Jelly bush honey and Jungle honey,
etc.), and the varieties are due to components of the flower sources. One
variety, Jungle honey, is collected from timber and blossom by wild honey
bees that live in the tropical forest of Nigeria.
The jungle honey is produced by the African wild bees
(Apis mellifera adansonii) in the virgin forest of the Nsukka district, Enugu state of Nigeria. The bees usually have
their nests (the honey combs) inside the hollow of tree trunks. Organic
agriculture methods are generally practiced in and around the forest areas. The
main harvest period for jungle honey is usually between January -April. The
harvest time is during the night or very early morning, when the bees
activities are low.
1) Pentaclethra macrophyllaiUgba)
2) Parinari excelsa (Inyi)
3) Vitex doniana (Uchakiri )
4) Dialium guineense (Icheku)
5) Chrysophyllum albidum ( Udara)
6) Irvingia gabonensis ( Ogbono)
7) Mangifera indica (Mango)
Elaeis guineensis( Nkwu)
Raffia farinifera (Nkwo)
Some trees/plants were found in the jungle honey.